Single Channel FI System
Since many assays use several reagents that must be added in a given sequence, FI systems employ peristaltic pumps that propel carrier and reagent streams to merge at confluence points. This flow geometry allows reagents to be added continuously to the injected sample at a desired concentration, so that reactions can be carried out in sequence as the sample zone passes through the first and second reactor. A majority of FI systems use peristaltic pumps that allow flow rates of carrier and reagents to be controlled by choosing the pump revolution rate, and by selecting pump tubes of a desired diameter. A typical flow rate is 0.5 -2.0 mL/min of individual streams, while the injected volume is usually in the range of 50 to 300mL.
An alternative to peristaltic pump is a MSFIA analyzer built around a multi syringe .
1.3.1.
